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Journal: Research
Article Title: A pH-Responsive Polysaccharide Hydrogel Modulates Oxygen Delivery and Immunometabolic Remodeling for Diabetic Bone Regeneration
doi: 10.34133/research.1428
Figure Lengend Snippet: GOZ6 improves mitochondrial function in BMSCs and promotes osteogenic differentiation. (A) Representative fluorescence images of JC-1 in BMSCs under different hydrogel treatments. Scale bar, 200 μm. (B) JC-1 flow cytometric analysis of BMSCs in different groups. (C) Representative fluorescence images of ROS staining in BMSCs under different hydrogel treatments. (D) Representative images of alkaline phosphatase (ALP) staining and Alizarin Red S (ARS) staining. Scale bar, 100 μm. (E) Quantitative analysis of ALP activity and mineralized nodule formation. (F) Immunofluorescent staining of BMSCs cultured for 3 d: green (COL1), red (phalloidin), and blue (DAPI). Scale bar, 200 μm. (G) Quantitative analysis of COL1 fluorescence intensity. (H to J) RT-qPCR analysis of osteogenesis-related gene expression ( Alpl, Runx2, and Col1a1 ) in BMSCs. Data are presented as the mean ± SD ( n = 5). Compared with the GO group: * P < 0.05, ** P < 0.01, *** P < 0.001, ns: not significant ( P > 0.05). Compared with the GOZ6 group: # P < 0.05, ## P < 0.01, ### P < 0.001, ns: not significant ( P > 0.05).
Article Snippet: Mitochondrial membrane potential was evaluated using a
Techniques: Fluorescence, Analysis, Staining, Activity Assay, Cell Culture, Quantitative RT-PCR, Gene Expression
Journal: Analytical Cellular Pathology (Amsterdam)
Article Title: HDAC4 Promotes Cancer Stemness in Nonsmall Cell Lung Cancer by Upregulating SLC38A2 Expression via HIF‐1α to Enhance Glutamine Metabolism
doi: 10.1155/ancp/2525721
Figure Lengend Snippet: Knocking down HDAC4 inhibits glutamine metabolism in A549 cells. (A) Glutamine uptake levels in A549 cells. (B) Glutamate production levels in A549 cells. (C) α‐Ketoglutarate production levels in A549 cells. (D) Measurement of GLS activity in A549 cells. (E) DCFH‐DA staining was used to measure the level of ROS in cells. (F) A kit was used to measure the GSH/GSSG ratio. (G) Western blotting was used to detect the expression of Nrf2, HO‐1, and NQO1. ∗∗ p < 0.01, ∗∗∗ p < 0.001. Data are presented as mean ± SD. All experiments were performed with three independent biological replicates. Statistical analysis was conducted using Student’s t ‐test for panels (A)‒(G). GLS, glutaminase; DCFH‐DA, 2 ′ ,7 ′ dichlorodihydrofluorescein diacetate; ROS, reactive oxygen species; GSH, reduced glutathione; GSSG, oxidized glutathione; Nrf2, nuclear factor erythroid 2 related factor 2; HO‐1, heme oxygenase 1; NQO1: NAD(P)H quinone dehydrogenase 1. The original, uncropped western blot images for Figure are provided in the Supporting Information.
Article Snippet: Intracellular ROS levels in A549 cells were detected using a
Techniques: Activity Assay, Staining, Western Blot, Expressing, Analysis